Efficacy of eco3spa Sanitising Tablets for prevention of bacterial growth

The graphs below show the bacterial growth of E-coli and Pseudomonas Aeruginosa measured as a function of the concentration of eco3spa Active Oxygen in water at both 25C and 37C and a range of pH values.

The dilution is equivalent to the number of tablets stated used in a 1200 litre hot tub.

The vertical axes represent the Optical Density of the sample. A higher Optical Density represents a higher bacterial growth. Calibration data is available in the full report. The calibration varies slightly between E-coli and Pseudomonas Aeruginosa and is a function of temperature but, broadly, an Optical Density of 0.2AU represents a log reduction of between 3.5 and 4 or a 99.97% - 99.99% reduction in potentially harmful bacteria.

The conclusion from the above results is that no measurable planktonic growth was seen for both E-coli and Pseudomonas Aeruginosa at any Active Oxygen concentration above 1 tablet dissolved in 1200 litres water at any pH between pH 7 and pH 8 at both temperatures.

A slight degradation in performance is seen in the cooler water at pH 8, but even in this case the planktonic growth is minimal.